Contact Information

  • Carl S. Lecher
  • Asst. Prof.Chemistry
  • School of Mathematics and Sciences
  • Marian College
  • 3200 Cold Spring Rd
  • Indianapolis, IN 46222
(317) 955 - 6005

A Greener Synthesis of Creatine

Laboratory Procedure (PDF)

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This simple procedure for the synthesis of creatine is a student favorite, especially for student athletes. The procedure demonstrates the preparation and isolation of a commonly known dietary supplement from sarcosine and cyanamide. Aqueous household ammonia functions both as the catalyst and the solvent. The experiment can be utilized for a variety of purposes within the organic chemistry curriculum, including the chemistry of nitriles, and is useful for starting a discussion of the impact of experimental design on the generation of impurities. Students typically achieve good to excellent yields (60 to 90%), with an average yield of 75%.

In other routes, creatine is typically synthesized from sarcosine and cyanamide as well. However, there are deficiencies in the usual routes. Cyanamide is used in excess (2 or more equivalents), concentrated ammonium hydroxide is used as the catalyst, and two equivalents of NaCl are used as an additive to aid in the precipitation of the product. Students should be able to identify three ways to potentially improve both the greenness and quality of the creatine synthesized by this method. The first is to reduce the amount of cyanamide to one equivalent. In addition to improving the effective mass yield of the reaction, it should greatly reduce the generation of the impurity dicyanodiamide, formed from the dimerization of cyanamide. The second is to eliminate use of NaCl. This would also improve the effective mass yield of the reaction, as well as eliminate the presence of any sodium chloride impurities in the procedure. The third would be to eliminate use of concentrated ammonium hydroxide, which presents a host of safety problems, including causing severe skin irritation, skin burns, and severe irritation to the upper respiratory tract. Replacing concentrated ammonium hydroxide with household ammonia (which can be further diluted) would eliminate these safety concerns and the need for a fume hood.

The link to the laboratory procedure includes a context specific scenario, background information, laboratory procedure, post- lab questions, and a result submission form.

Summary prepared July 2008 by Dr. Carl S. Lecher, School of Mathematics and Sciences, Marian College.


Lecher, C. S. A Greener Synthesis of Creatine, School of Mathematics and Sciences, Marian College, 2008

Category Descriptors

Chemistry Concepts
  • Addition Rxn
  • Aqueous Solution Chemistry
  • Catalysis
  • Equilibrium
  • Melting Points and Ranges
  • Natural Products
Laboratory Techniques
  • IR Spectroscopy
  • Melting Points and Melting Ranges
  • NMR Spectroscopy
  • Vacuum Filtration
Green Chemistry Principles
  • Design Less Hazardous Chemical Syntheses
  • Minimize the Potential for Accidents
  • Prevent Waste
  • Use Catalysts
  • Use Safer Solvents/Reaction Conditions
Chemistry Subdiscipline
  • Organic Chemistry
Target Audience
  • Colleges/Universities
  • Secondary Schools
  • Original Contributions - unpublished